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Exam 2 study guide(16)
Fundamentals of Microbiology (MCB 2610)
University of Connecticut
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Final Study Guide MCB2610 Fundamentals of Microbiology Exam 2 – Fall 2019 Note: This is an outline of the topics we have covered in class. You are also responsible for all material presented in lecture including all Power Point slides and any classroom discussions)
Study your notes for the 5 new microbe minutes. Make tables that allow you to compare specific features of the microbes (aka, Gram + vs. Gram -, spore former versus non, flagella versus none, pathogenic versus non, morphology so bacterial shape, cocci, rods etc.)(4 questions will come from this material)
Study your notes and slides from Dr. Sarah Hird’s presentation. Focus on general material. (4 questions will come from this material)
Read the assigned paper on Cow Birds. Focus on general material (4 questions will come from this material) Lecture Material Chapter 6A (from slide #32) (12 questions will come from this material)
Difference between complex and synthetic media
Complex media components and why they are important
Functional media types
Understanding differences between selective, differential and enriched media and some examples.
Why is it difficult to grow some organisms on artificial media and how can we identify organisms that can't be cultured on artificial media?
What is the difference between a streak plate, spread plate, and pour plate, and what are each used for?
Why do we use the term CFU's when counting colonies on a plate?
Measuring microbial population growth: know the different methods, how they work, think about what circumstances might make one method more suitable than another; know when we measure in CFUs vs. # of cells and why.
Know how to use counts to do calculations!! Example slide in our class powerpoint (count/dilution x volume plated) (simple calculations, no calculator required)
MPN (most probable number estimate) - I will NOT ask you to calculate MPN
Growth and the growth curve in Batch culture - know what a standard curve looks like, know all phases of the curve, know what is considered balance growth and what happens during shift up and shift down experiments.
Know what generation time, growth rate and growth yield are and how to determine simple calculations (no calculator required).
Know the reasons we get into stationary phase, the hypotheses for senescence, and some adaptations that allow cells to survive starvation (persister cells, VBNC).
Death and why growth doesn’t just plummet during the death phase.
Continuous vs batch culturing systems.
Importance of dilution rate in the chemostat and how that can influence growth
chemostat vs. turbidostat Lecture Material Chapter 6B (all slides) (14 questions will come from this material)
Difference between sanitization and sterilization
Difference between antiseptics and disinfectants
Difference between cidal and static agents
Conditions that influence the effectiveness of antimicrobial agents - what they are and why these specific conditions might change the effectiveness of an agent
Difference between physical and chemical control methods
Filtering - how it works, pros and cons, types of things that can be filter sterilized
Moist Heat - know the mechanism of killing and the 4 processes involving moist heat (boiling, pasteurization, steam sterilization, Tyndallization)
Dry Heat - know the mechanism of killing, how it differs from wet heat, and when dry heat is appropriate
UV/ionizing radiation-what situations each might be appropriate for
Classes of chemical agents (phenolics etc.), knowledge of their mechanism of killing and examples of each
Disk Diffusion assays for evaluating a disinfectant
Exam 2 study guide(16)
Course: Fundamentals of Microbiology (MCB 2610)
University: University of Connecticut
