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E.coli Lab Report
Course: Microbiology (BIOL 301)
12 Documents
Students shared 12 documents in this course
University: University of Maryland Eastern Shore
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Erika Martinez
Bio 223
Lab Report: The growth and transformation of E.coli
Introduction:
Bacteria are classified as prokaryotes. Prokaryotes are single-celled organisms such as
bacteria that have no distinct nucleus. (Introduction to Bacteria) A typical prokaryote is bound by
a plasma membrane and a cell wall. (Prokaryotes) The fact that the genetic material of bacteria is
contained in a single, circular chain of Deoxyribonucleic acid that is not enclosed with a nuclear
membrane. Deoxyribonucleic acid (DNA) is a natural polymer which encodes the genetic
information required for the growth, development, and reproduction of an organism. Found in all
cells, it consists of chains of units called nucleotides. E. Coli is bacteria. (Deoxyribonucleic Acid)
Eschenchia coil is the full genus and species name. Competent E. Coli cells are cells that are able
to pick up the plasma. We use competent E. Coli cells in our experiment because we want to the
E. coli to pick up extracellular DNA. Ampicillin is penicillin antibiotic to treat bacterial
infections. The reason why we use ampicillin is to select the bacteria that has the plasmid which
has the antibiotic resistance gene. A plasmid is a small DNA molecule within a cell that is
physically separated from a chrosome's DNA and can replicate independently. Transformation
involves the uptake of free or naked DNA released by donor by a recipient. (Introduction to
Bacteria) The purpose of this experiment was to observe the transformation and growth of E.
Coli cells on Luria broth and Luria broth with ampicillin.
Materials and Methods: