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ELN - Chromatography
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Course
(CHEM 2323, 2423) Organic Chemistry I (CHEM 227)
246 Documents
Students shared 246 documents in this course
University
Texas A&M University
Academic year: 2021/2022
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ELN - Analytical Technique
Chromatography
Purpose
Column chromatography is a technique applied to an experiment to purify compounds according
to their polar magnitude. To achieve successful column chromatography it is important to avoid
contamination.
Notes
- Ensure the there is liquid in the column at all times
- Do not allow the liquid to reach below the silica gel line, continue to refill
- Do not lose any of the liquid product dripping out
Protocol
Conditioning of the column
● Fill the top of the mini-column with 10%-90% ethyl acetate/hexane solvent.
● Obtain a pipet bulb and apply it to the top of the column, squeeze the bulb to force
solvent through the gel portion of the column
● When removing the pipet, maintain pressure and pull the bulb off to avoid inverse
pressure flow
● Do not allow the solvent level to drop below the silica line at the top
Was this document helpful?
ELN - Chromatography
Course: (CHEM 2323, 2423) Organic Chemistry I (CHEM 227)
246 Documents
Students shared 246 documents in this course
University: Texas A&M University
Was this document helpful?
ELN - Analytical Technique
Chromatography
Purpose
Column chromatography is a technique applied to an experiment to purify compounds according
to their polar magnitude. To achieve successful column chromatography it is important to avoid
contamination.
Notes
- Ensure the there is liquid in the column at all times
- Do not allow the liquid to reach below the silica gel line, continue to refill
- Do not lose any of the liquid product dripping out
Protocol
Conditioning of the column
● Fill the top of the mini-column with 10%-90% ethyl acetate/hexane solvent.
● Obtain a pipet bulb and apply it to the top of the column, squeeze the bulb to force
solvent through the gel portion of the column
● When removing the pipet, maintain pressure and pull the bulb off to avoid inverse
pressure flow
● Do not allow the solvent level to drop below the silica line at the top
