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Serology - Lectures on blood, bloodstains, and semen
Lectures on blood, bloodstains, and semen
Course
College Writing (Hon) (ENGL 1111)
6 Documents
Students shared 6 documents in this course
University
Northeastern University
Academic year: 2020/2021
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- Typing of Dried Blood Stains: Absorption-elution technique
- Antiserum on blood stain causes antibodies to mix with specific antigens
- Unrated serum is washed off
- Elution: breaking of antibody-antigen bond
- Known rbcs are added
- Agglutination will occur if antigens on RBCs were also on the stained material
- Differentiating Blood Types
- Enzymes: proteins that speed up or slow down chemical reactions
- Enzymes that exist in different forms are called polymorphic and can be broken down into their separate proteins (iso-enzymes)
- Phenotypes
- PGM: electrophoresis can separate the protein components into iso-enzymes
- EAP: determined by the intensity of the fluorescence
- The darker the band, the more it fluoresced
- Enzymes: proteins that speed up or slow down chemical reactions
- Bloodstain Spatter
- A blood droplet will remain spherical in the air
- Once a blood droplet impacts a surface, a bloodstain is formed
- Two droplets falling from the same height and angle and hitting the same surface will produce the same shape
- Bloodstains as evidence:
- Origins of bloodstain
- Distance of bloodstain from target
- Direction from which blood impacted
- Speed with which blood left its source
- Position and movement of victim and assailant
- Number of blows/shots
- Droplet Volume
- Directly dependent upon the place it originates from
- The impact area is called the target surface
- Volume is from 0.03-0 cc
- Shape is effected by size, angle, velocity, and texture
- On clean glass or plastic, the droplet will have smooth outside edges
- On rough surfaces, the droplet with have scalloped edges
- Bloodstains show:
- Movement and direction of a person or an object
- Number of blows/shots/etc.
- Type and direction of impact
- Semen
- Sperm
- One of the smallest and most highly specialized cells in the human body
- Has a head and a tail
- Contains 23 chromosomes w the genetic material found in the head
- Determination of seminal fluid:
- Acid phosphatase color test
- The enzyme secreted by the prostate gland into the seminal fluid will turn purple
- Will fluoresce under UV light
- Prostate Specific Antigen (PSA) or p
- P30 is isolated and injected into a rabbit where antibodies are produced
- Antigens and antibodies move toward each other
- Formation of a precipitation line shows presence of p
- Must be seminal fluid
- Acid phosphatase color test
- Sperm
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Serology - Lectures on blood, bloodstains, and semen
Course: College Writing (Hon) (ENGL 1111)
6 Documents
Students shared 6 documents in this course
University: Northeastern University
Was this document helpful?
- Typing of Dried Blood Stains: Absorption-elution technique
- Antiserum on blood stain causes antibodies to mix with specific antigens
- Unrated serum is washed off
- Elution: breaking of antibody-antigen bond
- Known rbcs are added
- Agglutination will occur if antigens on RBCs were also on the stained
material
- Differentiating Blood Types
- Enzymes: proteins that speed up or slow down chemical reactions
- Enzymes that exist in different forms are called polymorphic and can be
broken down into their separate proteins (iso-enzymes)
- Phenotypes
- PGM: electrophoresis can separate the protein components into
iso-enzymes
- EAP: determined by the intensity of the fluorescence
- The darker the band, the more it fluoresced
-
