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Platelet estimation lab1
Medical Laboratory Science (BS MLS 1)
Saint Louis University (Philippines)
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H E M A T O L O G Y P a g e | 1
PLATELET ESTIMATION Every laboratory derived their own platelet estimation
PBS Preparation:
- EDTA blood EDTA– anticoagulant of choice in platelet counting; preserve morphology of platelets; distinguishable platelets; no significant change in shape/appearance of platelets
- Glass slide
- Wright stain
Platelet count:
- Microscope
- Immersion oil Under OIO, choose zone of
morphology →
Count platelets in
10 fields →
Average of 10 OIFs x PEF
zone of morphology – area where you evaluate morphology of red cells; where we start differential count; where we start counting platelets PEF- platelet estimation factor
Average of platetels in 10 OIFs: must be whole number
Platelets: not perfectly circular bodies; may change in shape when outside
blood vessels; may show projections (may be normal/ abnormal – like in
microclots formation )
Describe plateltes:
Deep-blue to purple colored bodies Circular or with changed shape (but still counted) 1/9 the size of normal RBCs
PLATELET ESTIMATION FACTOR
Derived by correlating a machine count and a smear count 30 specimens from health subjects Machine count Prepare PBS for each Automation : cells are counted in the principle of their size Mean cell volume: RBC Mean platelet volume: platelet Make use of Coulter principle: body passes through a direct current direct current disturbed: disturbance is counted as an impulse 1 impulse: 1 cell Any increase in size of platelets: decrease the count of RBCs Any clot formation/aggregation of platelets (confused as bigger size): increase the count of RBCs
Count and record number of platelets in 10 consecutive fields
→
Divide the total number of platelets found by 10 to obtain average number per single OIF
= total number of platelets 10
→
For each specimen, divide the corresponding machine count by average number of platelets per OIF to obtain individual PEFs
= machine count average number of platelets per OIF
→
Add all individual PEF from 30 specimens and divide by 30 to obtain average PEF
=PEF1 + PEF2 + ⋯ PEF30 30
H E M A T O L O G Y P a g e | 2
⁂ Machine count – expressed in SI unit (150 = 150x10 9 /L); just copy ⁂ Smear count – average from 10 OIFs
iPEF =
MC
SC
PEF =
total iPEF 30
PEF =
528
30
= 𝟏𝟖
Sample problem: patient X need to obtain estimated platelet count
⁂ DO NOT SAY ABNORMAL/NORMAL: instead write ADEQUATE/DECREASED/INCREASED ⁂ We do not report (release results) of quantitative estimated platelet count – only report adequate/decreased/increased
Platelet estimation lab1
Course: Medical Laboratory Science (BS MLS 1)
University: Saint Louis University (Philippines)
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