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Physio Ex Exercise 8 Activity 1
Introduction to Human Anatomy and Physiology (BIOL 164)
University of Maryland Global Campus
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PhysioEx Lab Report
Exercise 8 : Chemical and Physical Processes of Digestion
Activity 1 : Assessing Starch Digestion by Salivary Amylase
Name: Pierre-Louis
Date: 8 March 2024
Session ID: session-bf628e67-df22-0e7e-95fc-9f3cb6bca03c
Pre-lab Quiz Results
You scored 0% by answering 0 out of 6 questions correctly.
The substrate for amylase is
You did not answer this question.
Correct answer: starch and carbohydrate.
1
Which of the following is true of enzymes?
You did not answer this question.
Correct answer: Their activity can be affected by temperature and pH.
2
The reagent IKI tests for the presence of
You did not answer this question.
Correct answer: starch.
3
Which of the following is not true of controls?
You did not answer this question.
Correct answer: A negative result with a positive control is required to validate the test.
4
Which of the following is an end product of starch digestion?
You did not answer this question.
Correct answer: maltose and glucose.
5
Hydrolases are enzymes that break down large molecules into smaller subunits through the addition of.
You did not answer this question.
Correct answer: water.
6
Experiment Results
Predict Question
Stop & Think Question
Experiment Data
Tube No
Reagent 1
Reagent 2
Reagent 3
Treatment Time Temp IKI Benedict's
1 Amylase Starch pH 7. Buffer Boiled 60 37 + -
2 Amylase Starch pH 7. Buffer Frozen 60 37 - ++
3 Amylase Starch pH 7. Buffer None 60 37 - ++
4 Amylase
Deionized Water
pH 7. Buffer None 60 37 - -
5
Deionized Water Starch pH 7. Buffer None 60 37 + -
6
Deionized Water Maltose pH 7. Buffer None 60 37 - ++
7 Amylase Starch pH 2. Buffer None 60 37 + +
8 Amylase Starch pH 9. Buffer None 60 37 + +
Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
Predict Question: What effect do you think boiling and freezing will have on the activity of the amylase enzyme?
Your answer: Boiling will decrease amylase activity and freezing will have no effect.
1
Tube 2 (amylase, starch, pH 7 buffer) appears to have the same amount of starch digested as tube 3 (amylase, starch, pH 7 buffer) because
You correctly answered: freezing had no effect on the enzyme.
1
Tubes 3 (amylase, starch, pH 7 buffer), 7 (amylase, starch, pH 2 buffer), and 8 (amylase, starch, pH 9 buffer) reveal that
You correctly answered: amylase activity was highest at pH 7.
1
Your answer:
Using a 37 degree incubation temperature to test salivary amylase activity is important since
37 degrees is the same temperature as the body. This shows that temprature is the most
ideal for amylase activity.
Physio Ex Exercise 8 Activity 1
Course: Introduction to Human Anatomy and Physiology (BIOL 164)
University: University of Maryland Global Campus
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